一、个人简介

高嵩，男，教授，硕士生导师，南京林业大学、上海海洋大学兼职博士生导师。入选江苏省333高层次人才、江苏省六大人才高峰、江苏省青蓝工程中青年学术带头人和连云港市521高层次人才，获评江苏省高校优秀共产党员、江苏省归侨侨眷先进个人、连云港市十大最美科技创新之星和江苏海洋大学优秀研究生导师。主持国家自然科学基金2项及多项省、市、厅级科研项目，获国家技术发明奖二等奖1项。以第一作者或通讯作者发表研究论文40余篇，其中包括Nature Communications、PNAS、Nucleic Acids Research、ACS Sustainable Chemistry & Engineering、Analytical Chemistry、Journal of Agricultural and Food Chemistry、Sensors and Actuators: B. Chemical、Aquaculture、Food Microbiology等知名SCI期刊论文。获授权发明专利6件，指导学生获评江苏省优秀硕士学位论文1篇。

E-Mail:gaos@jou.edu.cn

通讯地址：江苏省连云港市苍梧路59号

二、研究方向

生物催化和绿色合成、生物传感和分子检测

三、代表性成果

1.论文

[1]Yukang Chen, Guangxi Yu, Hongtao Kang, Jiangyuan Liu,Song Gao*, Zhiguo Dong*, Pei Wang* (2025) Duplex in-one-tube detection of two important shrimp farming pathogens with color indication using a method combining RPA and PfAgo.Aquaculture, 597, 741949.

[2]Song Gao*, Miao Xu, Bo Guo, Bo Luo, Xiaofu Shao, Yue Wang, Yang Cao, Di Zhang, Xinxin Ban, Lisha Wei, Pei Wang* (2024) Site-directed immobilization of an engineered cytidine deaminase on a magnetic carrier with bacteriophage-aided nanosurface for multiround biocatalysis.ACS Sustainable Chemistry & Engineering, 12, 13438-13451.

[3]Chenjie Zhao, Yixin Tang, Miao Xu, Yue Wang, Bo Luo, Pei Wang*,Song Gao*(2024) A highly sensitive on-site duplex genotyping method dRPG for simultaneous detection of SARS-CoV‑2 key mutations with single nucleotide resolution.Sensors and Actuators: B. Chemical, 418, 136238.

[4]Bo Guo, Lihong Yang, Yu Wang, Chenjie Zhao, Xue Zhang, Yixin Tang, Yue Wang, Hui Shen*,Song Gao*, Pei Wang* (2023) Pyrococcus furiosus Argonaute with isothermal amplification for fast and ultra-sensitive diagnosis of acute hepatopancreatic necrosis disease in shrimp.Aquaculture, 575, 739821.

[5]Wang P., Guo B., Zhang X., Wang Y., Yang G., Shen H.,Gao S.*, Zhang L.* (2023) One-pot molecular diagnosis of acute hepatopancreatic necrosis disease by recombinase polymerase amplification and CRISPR/Cas12a with specially designed crRNA.Journal of Agricultural and Food Chemistry, 71, 6490-6498.

[6]Wang P., Yang L., Guo B., Shang Z.,Gao S.*, Zhang X.* (2023) Highly sensitive detection of white spot syndrome virus with an RPA-CRISPR combined one-pot method.Aquaculture, 567, 739296.

[7]Yang L., Guo B., Wang Y., Zhao C., Zhang X., Wang Y., Tang Y., Shen H., Wang P.,Gao S.*(2023) Pyrococcus furiosus Argonaute combined with recombinase polymerase amplification for rapid and sensitive detection of Enterocytozoon hepatopenaei.Journal of Agricultural and Food Chemistry, 71, 944-951.

[8]Wang P., Zhang X., Shen H., Lu Q., Li B.*, Liu X.*,Gao S.*(2023) A one-pot RPA-CRISPR detection method for point-of-care testing of Enterocytozoon hepatopenaei infection in shrimp.Sensors and Actuators: B. Chemical, 374, 132853.

[9]Zhao C., Yang L., Zhang X., Tang Y., Wang Y., Shao X.,Gao S.*, Liu X.*, Wang P.* (2022) Rapid and sensitive genotyping of SARS-CoV-2 key mutation L452R with an RPA-PfAgo method.Analytical Chemistry, 94, 17151-17159.

[10]Xu J., Yang Q., Wang P., Wu D., Yang X., Chen W.*,Gao S.*, Wang S.* (2022) Self-assembled β-galactosidase on T4 phage capsid through affinity binding with enhanced activity and stability for rapid bacteria detection.Sensors and Actuators: B. Chemical, 359, 131569.

[11]Yang X., Zhao P., Dong Y., Chen S., Shen H., Jiang G., Zhu H., Dong J.*,Gao S.*(2021) An isothermal recombinase polymerase amplification and lateral flow strip combined method for rapid on-site detection of Vibrio vulnificus in raw seafood.Food Microbiology, 98, 103664.

[12]Gao, S., Zhang, L., Rao, V. B. (2016) Exclusion of small terminase mediated DNA threading models for genome packaging in bacteriophage T4.Nucleic Acids Research, 44, 4425-4439.

[13]Sun, L.#, Zhang, X.#,Gao, S.#, Rao, P. A., Padilla-Sanchez, V., Chen, Z., Sun, S., Xiang, Y., Subramaniam, S., Rao, V. B., Rossmann, M. G. (2015) Cryo-EM structure of the bacteriophage T4 portal protein assembly at near-atomic resolution.Nature Communications, 6, 7548.

[14]Sun, S.#,Gao, S.#, Kondabagil, K.#, Xiang, Y., Rossmann, M. G., Rao, V. B. (2012) Structure and function of the small terminase component of the DNA packaging machine in T4-like bacteriophages.Proc Natl Acad Sci U S A, 109, 817-822.

2.专利

[1]中国发明专利.高嵩；赵陈洁;王佩;王昱;谷庆花.一种新冠病毒S蛋白E484Q突变的基因分型检测方法.（2025）专利号ZL 202211076140.8

[2]中国发明专利.高嵩；赵陈洁;王佩;杨丽红;张雪;唐怡鑫;王月.一种新冠病毒S蛋白L452R突变的基因分型检测方法.（2024）专利号ZL202211439079.9

[3]中国发明专利.高嵩；马超；廖磊；王佩；张雪；王昱.一种用于同时检测虾急性肝胰腺坏死症和肝肠孢子虫的多重RPA引物和探针组合物及检测方法.（2023）专利号ZL202110732960.7

[4]中国发明专利.高嵩；马超；王佩；孙涛；张雪；陈礼战；杨丽红.一种利用L/RPA快速检测SARS-CoV-2的探针及引物组、试剂盒、检测方法.（2021）专利号ZL202110507195.9

[5]中国发明专利.高嵩；尹欣；王佩；陈凯；韩挺翰；孙峰；许恒皓.一种利用甲基化酶的保护高效重组表达限制性内切酶的方法.（2020）专利号ZL201611048568.6（已转化）

[6]中国发明专利.高嵩;陶攀;许恒皓;刘伟;尹欣;程斌.使用细胞内同步表达法在T4噬菌体表面展示外源蛋白大分子的方法.（2014）专利号ZL201310033512.3（已转化）

3.获奖

黄和,任路静,纪晓俊,江凌,陈可泉,高嵩.生物法制备二十二碳六烯酸油脂关键技术及应用.国家技术发明奖二等奖, 2018.12.12.