一、个人简介

高嵩，生物学博士，江苏海洋大学教授、硕士生导师，南京林业大学、上海海洋大学兼职博士生导师。研究方向主要包括生物催化和绿色合成、生物传感和分子检测。以第一作者或通讯作者发表研究论文40余篇，其中包括Nature Communications、PNAS、Nucleic Acids Research、ACS Sustainable Chemistry & Engineering、Analytical Chemistry、Journal of Agricultural and Food Chemistry、Sensors and Actuators: B. Chemical、Aquaculture、Food Microbiology等知名SCI期刊论文，并申请发明专利多项。主持国家自然科学基金2项及多项省、市、厅级科研项目。获2018年国家技术发明二等奖，入选江苏省“333高层次人才培养工程”，江苏省“六大人才高峰”高层次人才，江苏省“青蓝工程”中青年学术带头人和连云港市“521高层次人才培养工程”。获评江苏省高校优秀共产党员、江苏省归侨侨眷先进个人、连云港市“十大最美科技创新之星”和江苏海洋大学优秀研究生导师。

E-Mail: gaos@jou.edu.cn

通讯地址：江苏省连云港市苍梧路59号

二、代表性论文

[1] Yukang Chen, Guangxi Yu, Hongtao Kang, Jiangyuan Liu, Song Gao*, Zhiguo Dong*, Pei Wang* (2025) Duplex in-one-tube detection of two important shrimp farming pathogens with color indication using a method combining RPA and PfAgo. Aquaculture, 597, 741949.

[2] Song Gao*, Miao Xu, Bo Guo, Bo Luo, Xiaofu Shao, Yue Wang, Yang Cao, Di Zhang, Xinxin Ban, Lisha Wei, Pei Wang* (2024) Site-directed immobilization of an engineered cytidine deaminase on a magnetic carrier with bacteriophage-aided nanosurface for multiround biocatalysis. ACS Sustainable Chemistry & Engineering, 12, 13438-13451.

[3] Chenjie Zhao, Yixin Tang, Miao Xu, Yue Wang, Bo Luo, Pei Wang*, Song Gao* (2024) A highly sensitive on-site duplex genotyping method dRPG for simultaneous detection of SARS-CoV‑2 key mutations with single nucleotide resolution. Sensors and Actuators: B. Chemical, 418, 136238.

[4] Bo Guo, Lihong Yang, Yu Wang, Chenjie Zhao, Xue Zhang, Yixin Tang, Yue Wang, Hui Shen*, Song Gao*, Pei Wang* (2023) Pyrococcus furiosus Argonaute with isothermal amplification for fast and ultra-sensitive diagnosis of acute hepatopancreatic necrosis disease in shrimp. Aquaculture, 575, 739821.

[5] Wang P., Guo B., Zhang X., Wang Y., Yang G., Shen H., Gao S.*, Zhang L.* (2023) One-pot molecular diagnosis of acute hepatopancreatic necrosis disease by recombinase polymerase amplification and CRISPR/Cas12a with specially designed crRNA. Journal of Agricultural and Food Chemistry, 71, 6490-6498.

[6] Wang P., Yang L., Guo B., Shang Z., Gao S.*, Zhang X.* (2023) Highly sensitive detection of white spot syndrome virus with an RPA-CRISPR combined one-pot method. Aquaculture, 567, 739296.

[7] Yang L., Guo B., Wang Y., Zhao C., Zhang X., Wang Y., Tang Y., Shen H., Wang P., Gao S.* (2023) Pyrococcus furiosus Argonaute combined with recombinase polymerase amplification for rapid and sensitive detection of Enterocytozoon hepatopenaei. Journal of Agricultural and Food Chemistry, 71, 944-951.

[8] Wang P., Zhang X., Shen H., Lu Q., Li B.*, Liu X.*, Gao S.* (2023) A one-pot RPA-CRISPR detection method for point-of-care testing of Enterocytozoon hepatopenaei infection in shrimp. Sensors and Actuators: B. Chemical, 374, 132853.

[9] Zhao C., Yang L., Zhang X., Tang Y., Wang Y., Shao X., Gao S.*, Liu X.*, Wang P.* (2022) Rapid and sensitive genotyping of SARS-CoV-2 key mutation L452R with an RPA-PfAgo method. Analytical Chemistry, 94, 17151-17159.

[10] Xu J., Yang Q., Wang P., Wu D., Yang X., Chen W.*, Gao S.*, Wang S.* (2022) Self-assembled β-galactosidase on T4 phage capsid through affinity binding with enhanced activity and stability for rapid bacteria detection. Sensors and Actuators: B. Chemical, 359, 131569.

[11] Yang X., Zhao P., Dong Y., Chen S., Shen H., Jiang G., Zhu H., Dong J.*, Gao S.* (2021) An isothermal recombinase polymerase amplification and lateral flow strip combined method for rapid on-site detection of Vibrio vulnificus in raw seafood. Food Microbiology, 98, 103664.

[12] Gao, S., Zhang, L., Rao, V. B. (2016) Exclusion of small terminase mediated DNA threading models for genome packaging in bacteriophage T4. Nucleic Acids Research, 44, 4425-4439.

[13] Sun, L.#, Zhang, X.#, Gao, S.#, Rao, P. A., Padilla-Sanchez, V., Chen, Z., Sun, S., Xiang, Y., Subramaniam, S., Rao, V. B., Rossmann, M. G. (2015) Cryo-EM structure of the bacteriophage T4 portal protein assembly at near-atomic resolution. Nature Communications, 6, 7548.

[14] Sun, S.#, Gao, S.#, Kondabagil, K.#, Xiang, Y., Rossmann, M. G., Rao, V. B. (2012) Structure and function of the small terminase component of the DNA packaging machine in T4-like bacteriophages. Proc Natl Acad Sci U S A, 109, 817-822.

三、代表性专利

[1] 中国发明专利. 高嵩；马超；廖磊；王佩；张雪；王昱. 一种用于同时检测虾急性肝胰腺坏死症和肝肠孢子虫的多重RPA引物和探针组合物及检测方法.（2023）专利号ZL202110732960.7

[2] 中国发明专利. 高嵩；马超；王佩；孙涛；张雪；陈礼战；杨丽红. 一种利用L/RPA快速检测SARS-CoV-2的探针及引物组、试剂盒、检测方法.（2021）专利号ZL202110507195.9

[3] 中国发明专利. 高嵩；尹欣；王佩；陈凯；韩挺翰；孙峰；许恒皓. 一种利用甲基化酶的保护高效重组表达限制性内切酶的方法.（2020）专利号ZL201611048568.6

[4] 中国发明专利. 高嵩; 陶攀; 许恒皓; 刘伟; 尹欣; 程斌. 使用细胞内同步表达法在T4噬菌体表面展示外源蛋白大分子的方法.（2014）专利号ZL201310033512.3

四、科技奖励

黄和, 任路静, 纪晓俊, 江凌, 陈可泉, 高嵩. 生物法制备二十二碳六烯酸油脂关键技术及应用. 国家技术发明奖二等奖, 2018.12.12.

五、学术兼职

SCI期刊审稿人：

Analytical Chemistry

Food Control

Acta Biochimica et Biophysica Sinica

Foodborne Pathogens and Disease

Biomedical and Environmental Sciences

Journal of Fish Diseases